![]() Note: no account needed to view educational/reference materials.An excellent video to start with is this one: Be sure to look at the valuable links in that video description as well. Note the VALUABLE resource links under the download link on the QuPath page, including the forum, docs, YouTube channel, etc.We have a copy on the analysis computer if needed. Download QuPath from For best results, install it on a workstation, as opposed to an underpowered desktop or laptop.You're going to want to analyze your data in QuPath (free), which is based on ImageJ, but is designed for large images, including those from multiplex experiments.If you can't find an example of someone using your antibody on the ZellScanner, don't despair, email the Flow Core and we can assist you.Note that the Canopy ZellScanner Publications/Webinar Page also lets you search publications.Check out the methods section for a table of antibodies used, with concentrations, which you will use as a STARTING POINT for your project.Look for papers that use the ZellScanner, your tissue type (frozen or paraffin embedded), species, and tissue of interest (if possible).Do a PubMed search for papers using the following terms: multiplex imaging, chipcytometry (one word), etc.Antibody Resources/Guidelines (Easiest Way):.Webinars require a free account to access.Canopy ZellScanner Publications/Webinar Page.Canopy ZellScanner Tutorials and Guides.This is an EXCELLENT course on spectral flow cytometry by the University of Chicago's Cytology and Antibody Technology facility.Can even save an experiment template to save time setting up at the machine! Account is free. Cytek Cloud - Good place for spectral panel design for Auroras (all of ours are 5 laser).Cytek (Aurora) Biosciences Resource Page. ![]() ![]() Can design spectral panels here as well, but Cytek Cloud has nicer tools specific to spectral experiments. Very helpful for designing panels for specific machines. Get a free account as a member of Pitt.Also see Troubleshooting Guide at UChicago (skip down to bottom 25% of page or ctrl-f (command F on Mac) and search for "Troubleshooting.".Troubleshooting and Advanced Declogging (LSR II & Fortessa).By University of Chicago's Cytology and Antibody Technology Facility.Scroll down for video on how to use Diva, how to fill sheath, and troubleshooting guides.Intro to Advanced Flow Cytometry - Florian Weisel - Fall 2021.Introduction to Flow Cytometry - Aarika MacIntyre - 2021.introducing spectral flow cytometry, data analysis, and ways to clean up your experiments/data. UChicago (download Flow Basics PPT from here) Although at first it seems to go over many of the same topics as the ThermoFisher video, it then goes deeper.(Cytek recently bought Luminex, which owns Amnis, which makes the ImageStream.) 1 "slot" = 1 project or lab to work on experimental troubleshooting, experiment design, or data analysis with an expert from Cytek. Coming Soon: ImageStream Workshop, July 11-12, 2023 (6 slots open).Coming soon: ImageStream Advanced topic hybrid seminar, July 11, 2023, "lunchtime." Specifics will be announced when it gets closer.Topics are Experimental Design and Data Analysis (can influence somewhat if you sign up early enough and express your preferences.).ImageStream - Virtual (only) seminar on May 31st, 2023 at 12:00pm.Flow Cytometry Education Upcoming Seminar(s)
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